Journal: MicrobiologyOpen

Article Title: The lantibiotic gallidermin acts bactericidal against Staphylococcus epidermidis and Staphylococcus aureus and antagonizes the bacteria‐induced proinflammatory responses in dermal fibroblasts

doi: 10.1002/mbo3.606

Figure Lengend Snippet: The effects of gallidermin on viability, cytotoxicity and immune responses of dermal fibroblasts. Human primary dermal fibroblasts (50,000 cells/well) were untreated (−C) or treated with various concentrations of gallidermin (25–400 μg/ml) for 24 hr. Viability was determined as uptake of neutral red by viable cells (a). As a measure of cell cytotoxicity, the absorbance values of LDH release were measured and analyzed as the relative change from the negative control (nontreated fibroblasts) set to one (b). Secretion of the proinflammatory mediator CXCL 8 was determined with ELISA and showed a significant increase when fibroblasts were treated with 400 μg/ml gallidermin, whereas lower concentrations had no effects on CXCL 8 (c). IL ‐6 displayed a similar pattern, i.e. only 400 μg/ml gallidermin significantly elevated IL ‐6 (d). Data are presented as mean values with SEM indicated. Statistically significant differences were determined by using one‐way ANOVA with Bonferroni post hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001). n = 3

Article Snippet: CytoSelect LDH Cytotoxicity Assay Kit (Nordic BioSite, Täby, Sweden) was performed according to the manufacturer's protocol.

Techniques: Negative Control, Enzyme-linked Immunosorbent Assay

Journal: The EMBO Journal

Article Title: Secretion of mitochondrial DNA via exosomes promotes inflammation in Behçet's syndrome

doi: 10.15252/embj.2022112573

Figure Lengend Snippet:

Article Snippet: CytoSelect LDH Cytotoxicity Assay kit , CosmoBio , CBA‐241 , .

Techniques: Western Blot, Purification, Produced, Affinity Purification, Flow Cytometry, Confocal Microscopy, Electron Microscopy, Amplification, Recombinant, Blocking Assay, Adjuvant, Enzyme-linked Immunosorbent Assay, LDH Cytotoxicity Assay, Isolation, DNA Extraction, dsDNA Assay, Real-time Polymerase Chain Reaction, Plasmid Preparation, Cloning, Mutagenesis, Sequencing, Transfection, DNA Ligation, Fractionation